تأثير مستخلصات أربعة نباتات طبيعية على حياة وانقسام خلايا Caco-2 سرطان القولون من نوع

El-burai, Hadil R (2017) تأثير مستخلصات أربعة نباتات طبيعية على حياة وانقسام خلايا Caco-2 سرطان القولون من نوع. Masters thesis, الجامعة الإسلامية بغزة.

This is the latest version of this item.

[img] Text
122045.pdf

Download (4MB)

Abstract

Background: Cancer is an aggressive disease that if untreated leads to death. In Palestine, like any other country in the world, cancer is one of the most dangerous health problems affecting individual’s life. Conventional treatment protocols are not effective in all cases. Nowadays the aim of many medical research is to discover new safer and more effective anticancer agents from natural materials. The use of plant extracts with potential anticancer therapeutic effects might be particularly significant, especially in Palestine, which is rich in thousands of plant species known for their medical uses. Objective: The main objective of the present study is to assess the possible cytotoxic and antiproliferative effects of natural crude extracts of four medicinal plants locally available against Caco-2 cell line. Methodology: Extracts from the dried aerial and seeds parts of the plants were prepared employing 70% ethanol, chloroform and distilled water using Soxhlet apparatus. All extracts were prepared at various concentrations, then tested against Caco-2 cells to screen their cytotoxic and antiproliferative effects by using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT). DAPI stain was used for determining chromatin condensation in cells. The extracts, which gave the best effects, were tested on normal cells (lymphocyte). After that, the two extracts were tested in a combination manner with 5-Fluorouracil (5-FU). Results: The results of the present study revealed that most crude extracts, except ethanolic, chloroformic and aqueous extracts of Portulaca oleracea and aqueous crude extract of R. officinalis and A. sativa, exhibited concentration dependent antiproliferative effect against Caco-2 cells to various degrees. R. officinalis chloroformic extract and A. sativa ethanolic extract gave the best antiproliferative effects at 24 h with IC50 77 μg/ml and 67 μg/ml respectively. The results show that the effect of the crude extracts was much lower on normal cells (lymphocytes) as compared to cancer cells (Caco-2 cells). For combination studies, the combination index (CI < 1) was synergistic at all combinations. The dose reduction index is 2.4 and 23 times for R. officinalis and 5-FU, respectively. While, the dose reduction index is 1.8 and 14 times for A. sativa and 5- FU, respectively. Conclusions: Among the tested natural plants crude extracts R. officinalis chloroformic extract and A. sativa ethanolic extract gave the best antiproliferative effects against Caco-2 cells and have low effect on normal lymphocytes. In addition, synergistic activity of these extracts with commercial chemotherapy showed promising results. Further studies are required to determine the active components of these extracts

Item Type: Thesis (Masters)
Subjects: Q Science > QE Geology
Q Science > QR Microbiology
Depositing User: أ. دارين علي أحمد حمد
Date Deposited: 01 Mar 2021 11:58
Last Modified: 01 Mar 2021 11:58
URI: http://scholar.alaqsa.edu.ps/id/eprint/3891

Available Versions of this Item

Actions (login required)

View Item View Item