Isolation, Molecular Identification and Lab Evaluation of the Entomopathogenic Fungi; (Metarhizium sp. and Beauveria sp.) against the Red Palm Weevil Rhynchophorus ferrugineus

El-Hindi, Mahmoud W (2016) Isolation, Molecular Identification and Lab Evaluation of the Entomopathogenic Fungi; (Metarhizium sp. and Beauveria sp.) against the Red Palm Weevil Rhynchophorus ferrugineus. Masters thesis, الجامعة الإسلامية بغزة.

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Abstract

Background: Plant diseases generate challenging problems in commercial, agriculture and pose real economic threats. The red palm weevil (Rhynchophorus ferrugineus) (RPW) is one of the most destructive pests of palms in the world. Nowadays, control methods revolve around treatments based on chemicals, biotechnological systems using semichemicals or the development of the sterile insect technique and Biological control. Objectives: Our aim was to evaluate the entomopathogenicity of indigenous Beauveria bassiana and Metarhizium anisopliae against larvae and adults of R. ferrugineus. Methodology: B. bassiana & M. anisopliae taken from dead adults and dead larvae of R. Ferrugineus. Utilizing morphological analysis & molecular identification test by using PCR technique. Evaluation the efficiency of the isolated fungi under lab conditions and optimize it as biological control agent product after divided all adults and larvae into 4 groups. Incubation the adults RPW groups for 28 days and 6 days for larvae RPW groups. On another hand, the ability of treated RPW male to infect if the females was examined. All Data was examined by (Abbott's Formula) in this study. Results: Our results showed that the B. bassiana and M. anisopliae exhibited a good biological control agent against larvae and adults of RPW. The pathogenicity of the two most virulent isolates and the toxicity assay on larvae showed the highest mortality percentage which reached to 100% against the larvae with B. Bassiana, but reaches to 90% after spraying the larvae with M. anisopliae, and reaches 43.3% after treated by pesticide. The bioassay on the adults of RPW and the maximum mortality of weevils reaches 100% on 28th day after spraying the adult with B. bassiana, while the mortality was up to 90% after spraying the adult with M. anisopliae. The mortality for the adults treated with pesticide arrives to 50% and the control group 10% at the same time. Also, our results revealed that the infection males of RPW by EPF can be disseminated into the healthy population, after treatment the male adults of the RPW by B. bassiana and M. anisopliae. The highest mortality of up to 90% for two isolated fungi compare with the group which was treated with pesticide (20%) after incubation for 28 day. Conclusions: Our research concludes that B. bassiana and M. anisopliae locally isolated can be used as biological control agents with great efficacy.

Item Type: Thesis (Masters)
Subjects: Q Science > QE Geology
Q Science > QR Microbiology
Depositing User: أ. دارين علي أحمد حمد
Date Deposited: 01 Mar 2021 11:57
Last Modified: 01 Mar 2021 11:57
URI: http://scholar.alaqsa.edu.ps/id/eprint/3868

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