CTLA-4 Gene Polymorphisms in Women with Idiopathic Recurrent Pregnancy Loss

Helles, Eman N (2016) CTLA-4 Gene Polymorphisms in Women with Idiopathic Recurrent Pregnancy Loss. Masters thesis, الجامعة الإسلامية بغزة.

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Abstract

Background: Recurrent pregnancy loss (RPL) is the miscarriage of two or more consecutive pregnancies before 20th gestational week. We suggest that dysregulated immune-tolerance contributes to idiopathic RPL. Cytotoxic T lymphocyte associated antigen-4 (CTLA-4) is considered as a negative regulator of T cell activation and its role in maintaining immune-tolerance is well established. Objective: The present study aimed to investigate the CTLA-4 +49 A/G , -1661 A/G, -318 C/T and -1722 T/C single nucleotide polymorphisms (SNPs) and predisposition to RPL in Gaza Strip - Palestine. Methods: This case-control study was performed on DNA samples from 200 women with a history of two or more pregnancy losses (case group) and 200 control women with at least two live births and without any previous history of RPL. PCR-based restriction fragment length polymorphism (RFLP-PCR) method was used for genotyping CTLA-4 polymorphisms. Results: Our study results revealed that there is no significant association between the allele/genotype frequencies of the investigated CTLA-4 SNPs and RPL. This relation remained true under dominant, co-dominant and recessive models. The A\G genotype of -1661 A\G polymorphism was higher in patient (45%) as compared to controls (39.5%) but without statistical significance. The minor allele frequencies (MAFs) of the CTLA-4 gene polymorphisms in the control group were as follows: +49A\G: 0.22, 318 C\T: 0.11, -1661 A\G: 0.26 and -1722T\C: 0.08. Conclusion: The study showed that there is no significant association between the four investigated CTLA-4 polymorphisms and the risk of RPL in the study population. Testing other CTLA-4 gene polymorphisms and the level of CTLA-4 expression in RPL patients is recommended. Key words: Recurrent pregnancy loss, CTLA-4, Gene Polymorphism, PCR-RFLP.

Item Type: Thesis (Masters)
Subjects: Q Science > QE Geology
R Medicine > R Medicine (General)
Depositing User: أ. دارين علي أحمد حمد
Date Deposited: 01 Mar 2021 11:51
Last Modified: 01 Mar 2021 11:51
URI: http://scholar.alaqsa.edu.ps/id/eprint/3770

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