Anticancer Effects of New Heterocyclic Compounds on Cervical Cancer Cells in Vitro

Alqaddi, Hala Ismaeel (2018) Anticancer Effects of New Heterocyclic Compounds on Cervical Cancer Cells in Vitro. Masters thesis, الجامعة الإسلامية بغزة.

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Abstract

Background: Cancer is a serious disease that is a burden on individuals and communities. Although many treatments are currently used to treat cancer, they are faced with many obstacles as cancer resistant which reduces the treatment efficacy. Of these cancers, cervical cancer still a leading cause female’s death worldwide. Recently, imidazo[1,2-a]pyridines compounds exhibited a lot of promise to as anticancer agents. Important examples of these compounds are currently at clinical trials. Objectives: This research was conducted to evaluate anticancer activity of newly synthesized imidazo[1,2-a]pyridines (Pico) compounds named 3-[(4-Chlorophenyl)diazenyl]-2,7- dimethylimidazo[1,2-a]pyridine 8 (Pico4) and 3-[(4-Chlorophenyl)diazenyl]-2,5- dimethylimidazo[1,2-a]pyridine 10 (Pico6) against cervical cancer cell line. Methodology: The cytotoxic and anti-proliferative effects of imidazo[1,2-a]pyridines (Pico) compounds were determined aganist HeLa cell line using MTT assay, trypan blue, scratch assay and Clonogenic assay. Western blot analysis was carried out to detect specific proteins and to determine the mechanism of action of Pico compounds. Results: Our results showed that the imidazo[1,2-a]pyridines (Pico) compounds named Pico4 and Pico6 have a strong anti-growth effect in time and concentration dependent manner in vitro against cervical cancer cell line. The cytotoxic effect of Pico4 against cervical cancer cell line was slightly more potent with IC50 value of 37.8 μM than Pico6 with IC50 value of 42 μM. Trypan blue assay results showed that both compounds induce cell death and anti-proliferative effect in HeLa cell line. Furthermore these compounds induce anti-migration effect where they inhibit cell migration by more than 35% as observed by wound healing assay. Clonogenic survival assay showed that Pico4 reduced HeLa survival rate more than 80% at high cell concentrations. The mechanism by which Pico4 and Pico6 induced cancer cells death by apoptosis as noted by the increasing level of PARP cleavage and increasing levels of P53 in the treated cells. Conclusion: The new imidazo[1,2-a]pyridines (Pico 4 and 6) compounds represent potential active anticancer compounds against cervical cancer cells in vitro. Keywords: Cervical cancer, imidazo[1,2-a]pyridines, biological activities, cytotoxicity, apoptosis

Item Type: Thesis (Masters)
Subjects: Q Science > QE Geology
Depositing User: أ. دارين علي أحمد حمد
Date Deposited: 01 Mar 2021 11:30
Last Modified: 01 Mar 2021 11:30
URI: http://scholar.alaqsa.edu.ps/id/eprint/3746

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