التأثير الضد بكتيري والضد بيوفيلمي لبعض المستخلصات النباتية و تأثيرها التآزري مع المضادات الحيوية

Abu Madi, Yasmeen Mosa (2018) التأثير الضد بكتيري والضد بيوفيلمي لبعض المستخلصات النباتية و تأثيرها التآزري مع المضادات الحيوية. Masters thesis, الجامعة الإسلامية بغزة.

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Abstract

Background: Antibiotics are important to inhibit bacterial activity, but some bacteria have the ability to adapt with environment and develop themselves, their ability to form biofilm, and their resistance to antibiotics. Therefore, alternative sources of antibiotics have to be found to inhibit bacterial activity. Objective:The aim of the study was to assess the antibacterial and antibiofilm effect of some medicinal plant extracts and their synergistic with antibiotic against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and klebsiella pneumonia. Materials: The medicinal plants that have been studying its effect against some clinically isolated bacteria is Glycyrrhiza glabra roots, Laurus nobili, Malus domestica peels, Melissa officinalis and Lagenaria siceraria peels. Clinical isolated bacteria used in the study are Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and klebsiella pneumonia. Methods:The extract of medicinal plants were prepared using microwave method for ethanolic extract and aqueous extracts. The antibacterial activities of extracts were confirmed using the disk diffusion method and the well diffusion method; the inhibitory zones were recorded in millimeters. The minimal inhibitory concentration (MIC) of the plant extracts were confirmed using microdilution method. The synergistic effect between plants extracts and antibiotics was assessed using disk diffusion method. Antibiofilm activity was confirmed by using microtitre dish against S. aureus and P. aeruginosa. Results: The results exhibit that there is a decrease in MIC of aquatic extract of M. domestica peels against E. coli (1.56mg/ml), and the ethanolic and aquatic extract of G. glabra roots, L. siceraria peels and M. officinalis against S. aureus 25 mg/ml, and the ethanol extract of G. glabra roots against P. areuginosa varying from 12.5 to 6.25 mg/ml. The MIC for aquatic extract of Glycyrrhiza glabra roots and L. nobilis showed 25 mg/ml against K. pneumonia. The MIC for ethanolic extract of M. officinalis and M. domestica peels was 25 mg/ml against K. pneumonia. The MIC for the ethanolic and aquatic extract of L. siceraria peels was 25 mg/ml against k. pneumonia. The results showed that MBC of ethanolic and aquatic extract of G. glabra roots against E. coli at 200 mg/ml. The MBC of ethanolic extract of G. glabra roots against S. aureus at 200 mg/ml. The MBC of aquatic extract of M. officinalis against P. aeruginosa at 200 mg/ml. The MBC of ethanolic and aquatic extract of M. officinalis against K. pneumonia at 200 mg/ml. The results of this study have shown that L. nobili aquatic extract concentration of 12.5mg/mL is which showed the highest inhibition on S. aureus biofilm formation 86.7%. M. domestica peels ethanolic extract concentration of 25mg/mL is the highest inhibition (90%) on P. aeruginosa biofilm formation. Conclusions:The plant extracts showed strong antibacterial and synergistic effect with most antibiotics against E. coli, S. aureus, P. aeruginosa and K. pneumonia. The antibiofilm effect against S. aureus and P. aeruginosa was cleary showen. Key words: Anti-biofilm, plant extracts, antibacterial effect and synergistic effect

Item Type: Thesis (Masters)
Subjects: Q Science > QE Geology
Depositing User: أ. دارين علي أحمد حمد
Date Deposited: 01 Mar 2021 11:29
Last Modified: 01 Mar 2021 11:29
URI: http://scholar.alaqsa.edu.ps/id/eprint/3731

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