In vitro inhibition of human leukemia THP-1 cells by Origanum syriacum L. and Thymus vulgaris L. extracts.

Ayesh, Basim and Abed, Abdalla A and Faris, Doa'a M (2014) In vitro inhibition of human leukemia THP-1 cells by Origanum syriacum L. and Thymus vulgaris L. extracts. BMC research notes, 7. p. 612. ISSN 1756-0500

[img] Text
1756-0500-7-612

Download (145kB)

Abstract

BACKGROUND Natural products including, traditional medicinal plants have emerged as a tempting alternative to conventional chemotherapeutic protocols of leukemia because of their minimum side effects and less documented drug resistance. METHODS Ethanol extracts were prepared from Thymus vulgaris L. and Origanum syriacum L. plants and investigated against the THP-1 leukemia cell line and freshly isolated peripheral blood mononuclear cells (PBMCs). The 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay and the lactate dehydrogenase (LDH) assay were respectively used to determine the cellular viability and cytotoxicity in response to treatment with increasing extract concentrations. RESULTS Both extracts exhibited a concentration dependent reduction in viability of the THP-1 cells (IC50 = 2.126 mg/mL for O. syriacum, and 0.1569 mg/mL for T. vulgaris). O. syriacum was more potent against the PBMCs (IC50 = 0.4247 mg/mL), while T. vulgaris was moderately selective (IC50 = 0.3345 mg/mL with PBMCs and SI = 2.1). Only in O. syriacum the reduction in cells viability was caused by cytotoxic effect against leukemic cells (LC50 = of 9.646 mg/mL). CONCLUSION T. vulgaris and O. syriacum are both antileukemic in vitro. T. vulgaris represents a potential selective cytostatic and safe target for future anticancer agents' development. O. syriacum on the other hand is cytotoxic against the leukemia cell line THP-1.

Item Type: Article
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Medicine, Health and Life Sciences > School of Biological Sciences
Depositing User: د. باسم محمد حسين عايش
Date Deposited: 25 Mar 2018 16:34
Last Modified: 25 Mar 2018 16:34
URI: http://scholar.alaqsa.edu.ps/id/eprint/339

Actions (login required)

View Item View Item