الكشف عن الجينات التي لها دور في إحداث الإمراضية لدى بكتيريا المكورات العنقودية الذهبية المقاومة للميثيسيلين والمعزولة من التجويف الأنفي

El-Hallaq, Maha M (2018) الكشف عن الجينات التي لها دور في إحداث الإمراضية لدى بكتيريا المكورات العنقودية الذهبية المقاومة للميثيسيلين والمعزولة من التجويف الأنفي. Masters thesis, الجامعة الإسلامية بغزة.

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Abstract

Background: Healthcare workers (HCWs) colonized with Staphylococcus aureus may serve as a reservoir of infection. This study was carried to determine the prevalence of MRSA and genetic make-up of S. aureus nasal colonizers in HCWs. Methodology: Nasal swabs were obtained from 202 HCWs and 205 NHCWs and molecular characterization of identified methicillin resistance S. aureus isolates was carried out using Polymerase chain reaction technique for leukocidin toxin, pyrogenic toxins and toxic shock syndrome toxin. Results: seventy-four HCWs (36.6%) were colonized with S. aureus (MSSA = 51; MRSA = 23). Thus the overall MRSA carriage rate was 11.3 % (n/N = 23/202) and 31.1% (n/N = 23/74) of those colonized with S. aureus harboured MRSA. There was statistically significant difference in S. aureus carriage rate between HCWs and NHCWs (P = 0.003). Most of the 74 isolates harbored at least one of the 13 enterotoxin gene (s). The most prevalent enterotoxin was seg (63.5%), followed by sei (55.4%),sea (45.9%), seb (36.4%),see (9.4%) and sej (5.4%). Considerable genetic diversity was detected. The present study revealed the relatively high rate of tst (48.6%),eta (44.5%) and luk (31%) as well as the wide distribution of enterotoxin genes among colonizing S. aureus. The highest combination between positive mecA isolates and other virulence gene was found in mecA-seg (41.8%), mecA-sei (40.5%) and mecA-sea-seb-seg-sei (24.3%). Conclusion: These findings provide insight into the virulence gene profiles of S. aureus nasal carriage among the HCWs and NHCWs over around Gaza strip. Keywords: Methicillin resistance Staphylococcus aureus, Oxacillin, Antibiotic susceptibility test, Staphylococcus enterotoxins, virulence associated genes.

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