المكافحة الحيوية للفيتوفثورا انفستنس في البطاطس باستخدام فطر الترايكوديرما فيريدي تحت ظروف المختبر والدفيئة في قطاع غزة

Mosleh, Farida N. (2019) المكافحة الحيوية للفيتوفثورا انفستنس في البطاطس باستخدام فطر الترايكوديرما فيريدي تحت ظروف المختبر والدفيئة في قطاع غزة. Masters thesis, الجامعة الإسلامية بغزة -عمادة البحث العلمي والدراسات العليا ماجستير تكنولوجيا حيوية.

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Abstract

Background: Potato late blight caused by Phytophthora infestans (Mont.) de Bary is one of the most destructive diseases worldwide. P. infestans can infect many members of Solanaceae family. The most economically important hosts are potato and tomato. It is becoming difficult to control this pathogen, which leading to more intensive use of agro-chemical. In the recent years, the environmental pollution caused by excessive use of chemical fungicide increased which lead to negative effect on human health and environment. Bio-control agents are considered environmentally safe alternatives for agro-chemical in controlling plant diseases. Objectives: The main objective of this study is to biological control Potato late blight caused by P. infestans using the eco-friendly fungus Trichoderma viride under laboratory and greenhouse conditions in Gaza strip. Methodology: P. infestans was isolated from infected potato plant and T. viride was isolated from agricultural soil in Gaza strip. These fungi were identified depend on their macroscopic and microscopic characteristics. Dual culture method and Poison agar technique were used to evaluate of the bio-control agent T. viride and the chemical fungicide (Ridomil Gold) against P. infestans in vitro. In addition, the effect of T. viride and chemical fungicide under greenhouse condition on disease intensity and selected plant growth parameters were evaluated. Result: In vitro, spores suspension of T. viride 6 ×108 spores/ml showed faster growth than P. infestans. After 4 days, the mycelia of T. viride overgrew the mycelia of P. infestans and destroyed it completely after 6 days of incubation. Culture filtrate of T. viride showed highly significant percent inhibition on dry weight of mycelia of P. infestans by 98.1% compared to broth media with fungicide (31%). In the greenhouse experiment, plants treated with T. viride as a protective agent hindered the infection with P. infestans by 100% and as therapeutic agent had significantly stopped the spread of the infection by (98-100)% , while the chemical fungicide had reduced the disease severity by 70% as protective agent and 50% as therapeutic agent. Conclusions: T. viride showed an excellent ability in controlling P. infestans under laboratory and greenhouse conditions and reached satisfactory levels in control late blight disease. Result from bioassays in this study proved the ability of T. viride to perform multiple mechanisms for controlling potato late blight disease. T. viride increased immunity of plant through enhanced plant growth, inhibited P. infestans and stimulated plant defense mechanisms Keywords: Potato late blight, Phytophthora infestans, Trichoderma viride, biological control and chemical fungicide (Ridomil Gold).

Item Type: Thesis (Masters)
Subjects: Q Science > Q Science (General)
Depositing User: أ. دارين علي أحمد حمد
Date Deposited: 23 Nov 2020 11:23
Last Modified: 23 Nov 2020 11:23
URI: http://scholar.alaqsa.edu.ps/id/eprint/2636

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