Isolation and Characterization of Streptococcus mutans as Causative Agent of Dental Caries in Gaza Strip and their Antibacterial Susceptibility Pattern

Abu Ismail, Ibrahim (2017) Isolation and Characterization of Streptococcus mutans as Causative Agent of Dental Caries in Gaza Strip and their Antibacterial Susceptibility Pattern. Masters thesis, Al Azhar University Gaza.

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Abstract

Background: Dental caries is a well-known major oral health problem in most countries. The multifactorial etiology of the disease includes multiple bacterial species, S. mutans is the main pathogen associated with the disease. S. mutans is a facultative gram-positive anaerobe commonly found in oral cavity. Objective: The aim of this study is to isolate and characterize S. mutans as potential causative bacteria of dental carries and studying its sensitivity to antibacterial agents and antibacterial mouth rinses from patients visit the dental clinic in Gaza strip. Material and method: Open label experimental study was performed on 300 patients and 300 control visit the dental clinics in Gaza strip. The study was carried out during the period from July 2015 to July 2016. All suspected S. mutans isolates were identified biochemically, tested against ten common used antibiotics by disc diffusion method, studied in vitro for efficacy of nine essential oils, five toothpaste and four mouth rinses. Results: Among 300 patients tested in this study, 95 showed a positive result for the presence of S. mutans in their saliva and dental caries. The most positive cases for the presence of S. mutans in dental caries and saliva were detected in the age group 20-35 year and in nonsmoker patients. Colgate showed the best antimicrobial activity among 5 different toothpastes. Antibiotic sensitivity test indicated that S. mutans was most susceptible against vancomycin (100%), tetracycline (83.2%), doxycycline (84.2%), ciprofloxacin (81.1%) and amoxyclav (75.8%). Gargarol was found to be the most effective mouth rinse XIII against S. mutans. Clove bud, lemon grass and Tea tree were the most effective oils against S. mutans respectively. Conclusion: S. mutans isolates were moderately resistant to antibiotics. Use of plant extracts (essential oils) may be recommended as a supportive or alternative option to conventional formulations. Key words: Streptococcus mutans, Dental caries, Gaza strip, Essential oils, Tooth paste, Mouth rinses.

Item Type: Thesis (Masters)
Subjects: Q Science > Q Science (General)
Depositing User: أ. طارق زياد عبد حنونة
Date Deposited: 18 Aug 2020 08:38
Last Modified: 18 Aug 2020 08:38
URI: http://scholar.alaqsa.edu.ps/id/eprint/2006

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